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KMID : 0380219940270060514
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Journal of Biochemistry and Molecular Biology
1994 Volume.27 No. 6 p.514 ~ p.521
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Effects of Thiol Group Inhibitors on Oxidant Production, Acid Phosphatase Release, and the Intracellular Ca2+ Level in Bovine Neutrophils
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Abstract
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Abstract:
@EN The effects of thiol group inhibitors on respiratory burst, degranulation, and intracellular Ca2+ level in bovine neutrophils were examine. Superoxide and HOCI production in arachidonic acid-activated neutrophils and NADPH oxidase activity
were
inhibited by HgCl2, PCMB, and PCMBSA, PMA-stimulated superoxide and H2O2 generation in neutrophils were inhibited by HgCl2, NEM, H-7 chlorpromazine, and propranolol, but were not affected by PCMB and PCMBSA. The inhibitory effect of NEM on
superoxide
generation in PMA-activated neutrophils was almost completely prevented by GSH and MPG. The activity of NADPH oxidase, which is obtained from arachidonic acid-activated neutrophils, was inhibited by PMA, and the inhibitory effect of PMA was
abolished by
HgCl2 and PCMb. Release of acid phosphatase from activated neutrophils was inhibited by HgCl2, NEM, PCMB, PCMBSA, EGTA, verapamil, and diltiazem, but was not affected by H-7, chlorpromazine, and propranolol. The arachidonic acid-induced elevation
of
Ca2+i was inhibited by HgCl2, NEM, EGTA, TMB-8, and verapamil, but was no affected by PCMB and PCMBSA. These results suggest that neutrophil responses, including superoxide generation and lysosomal enzyme release, may be influenced by the
oxidation
of
sulfhydryl groups. The protein kinase C-mediated neutrophil response and the intracellular calcium mobilization appear to be affected by a change of intracellular sulfhydryl groups.
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KEYWORD
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